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pet28a+ backbone  (Millipore)


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    Structured Review

    Millipore pet28a+ backbone
    Pet28a+ Backbone, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28a++backbone/pet28a/pmc11579443__NPH___244___2278___s004-161-24-26
    Average 90 stars, based on 1 article reviews
    pet28a+ backbone - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies
    Article Snippet: For replica moulding, approximately 20 gram of PDMS (Sylgard 184, Dow Corning) monomer and curing agent were mixed at a ratio 10:1, and degassed, poured onto the master and solidified overnight at 65 °C. .. Expression plasmids based on the pET28a backbone (Novagen) were constructed by Gibson Assembly . .. After bonding, microfluidic channels were flushed with 1% (v/v) trichloro(1H,1H,2H,2H-perfluoro-octyl)silane (Sigma-Aldrich) in HFE-7500 (3M Novec) to make them more hydrophobic.

    Construct:

    Article Title: Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies
    Article Snippet: For replica moulding, approximately 20 gram of PDMS (Sylgard 184, Dow Corning) monomer and curing agent were mixed at a ratio 10:1, and degassed, poured onto the master and solidified overnight at 65 °C. .. Expression plasmids based on the pET28a backbone (Novagen) were constructed by Gibson Assembly . .. After bonding, microfluidic channels were flushed with 1% (v/v) trichloro(1H,1H,2H,2H-perfluoro-octyl)silane (Sigma-Aldrich) in HFE-7500 (3M Novec) to make them more hydrophobic.



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